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Densitometric analysis of blots imaged with film. The same four chemiluminescent blots from Fig. 6 were then imaged with film (a). Blotting was performed using a mixture of rabbit- and mouse-derived primary antibodies to ADH and GAPDH, respectively, with the associated mixture of HRP-conjugated secondary antibodies. The average relative density of GAPDH (b) and ADH (c) was compared to the relative difference in μg quantity of protein load for the HeLa lysate (b) and the ng quantity of ADH-spike (c) between the triplicate replicates within each lane group over four blots. The fold difference in stain-free (SF) detected lane density for total protein and GAPDH was compared to that of the μg quantity of actual loading of HeLa lysate (d). A positive Pearson Correlation was obtained for total protein (SF) but not for GAPDH (p-values of 0.0398 and 0.274 respectively). d.
Free Download Film Semi Full 2013 Spike
Spikes are displayed as points in either two-dimensional (2D) or three-dimensional (3D) feature space, where a variety of manual, semi-automated or fully-automated clustering techniques can be applied to classify (sort) the spikes. Our spike sorting software can also perform spike extraction on continuously recorded neural data using a variety of different thresholding methods. OFS allows sorting verification through a variety of displays and can calculate cluster separation statistics and sort quality metrics. The sorted spikes can be exported in several formats for subsequent analysis.
Densitometric analysis of blots imaged with film. The same four chemiluminescent blots from Fig. 6 were then imaged with film (a). Blotting was performed using a mixture of rabbit- and mouse-derived primary antibodies to ADH and GAPDH, respectively, with the associated mixture of HRP-conjugated secondary antibodies. The average relative density of GAPDH (b) and ADH (c) was compared to the relative difference in μg quantity of protein load for the HeLa lysate (b) and the ng quantity of ADH-spike (c) between the triplicate replicates within each lane group over four blots. The fold difference in stain-free (SF) detected lane density for total protein and GAPDH was compared to that of the μg quantity of actual loading of HeLa lysate (d). A positive Pearson Correlation was obtained for total protein (SF) but not for GAPDH (p-values of 0.0398 and 0.274 respectively). d.
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